General Cell Based Assays
Meso Scale Discovery U-PLEX Mouse IL-10 ASY
The Meso Scale Discovery U-PLEX Mouse IL-10 Assay is an electrochemiluminescent immunoassay for the quantitative measurement of mouse interleukin 10 (IL-10) in serum plasma cell culture supernatants and other biological samples This assay utilizes MSD s proprietary SECTOR Imager and provides high sensitivity a broad dynamic range and requires low sample volume The lower limit of detection is 0 04 pg/mL This assay is for research use only and is not intended for diagnostic procedures The kit includes a 96-well plate coated with capture antibody detection antibody calibrator and assay diluent
Meso Scale Discovery U-PLEX Mouse IL-16 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-16 Assay is a multiplex immunoassay kit that enables simultaneous quantification of mouse interleukin 16 (IL-16) in serum plasma and cell culture supernatants This electrochemiluminescent assay utilizes MSD s proprietary SECTOR Imager and provides high sensitivity a wide dynamic range and requires low sample volume The kit includes a 96-well plate pre-coated with capture antibodies detection antibodies calibrators and controls Results are obtained in as little as 3 5 hours This assay allows for efficient reproducible analysis of IL-16 levels relevant to immunology and inflammation research
Meso Scale Discovery U-PLEX Mouse IL-6 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-6 Assay Kit is a sensitive immunoassay for detecting mouse interleukin 6 (IL-6) in various samples It uses MSD s electrochemiluminescent technology offering high sensitivity a wide dynamic range and quick results The kit includes a 96-well plate with capture antibodies for mouse IL-6 detection antibodies with an electrochemiluminescent label and calibrators for a standard curve Sample incubation is 2 hours with no wash steps quantifying IL-6 from 0 2-10 000 pg/mL
Eurofins Discoverx PathHunter eXpress CALCRL-RAMP1 (CGRP1) CHO-K1 β-Arrestin GPCR Assay, 2pl Kit. (93-0269E2CP0M)
The PathHunter CALCRL-RAMP1 (CGRP1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. β-CGRP) based activation of CALCRL GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CALCRL and EA-tagged β-Arrestin-2, along with untagged modulating protein RAMP1. Activation of CALCRL stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical 20S Proteasome Assay Kit
The 20S proteasome is the proteolytic core of a large protein degradation complex, the 26S proteasome. Proteasome inhibitors exhibit anti-inflammatory and antiproliferative effects, evidence that the proteasome may be an important drug target for the treatment for cancer and inflammatory diseases. Cayman’s 20S Proteasome Assay employs a specific 20S substrate, SUC-LLVY-AMC which, upon cleavage by the active enzyme, generates a highly fluorescent product with an emission wavelength at 480 nm.
Cayman Chemical Sphingomyelin Colorimetric As
Sphingomyelin is an important lipid component of cell membranes and lipoproteins. Cayman’s SM Assay provides a specific, sensitive, and convenient method for quantifying SM in plasma or serum in a 96-well format with a colorimetric readout at 595 nm.
Cayman Chemical Acid Phosphatase Colorimetric
Cayman’s Acid Phosphatase Assay provides a convenient method for detecting AP activity in plasma, serum, urine, and semen. The assay utilizes para-nitrophenyl phosphate (pNPP) as a chromogenic substrate for the enzyme. In the first step, AP dephosphorylates pNPP. In the second step, the phenolic OH-group is deprotonated under alkaline conditions resulting in p-nitrophenolate that yields an intense yellow color which can be measured at 405-414 nm. The kit provides all reagents needed to assay AP activity, and includes L-tartrate, an inhibitor of non-tartrate resistant APs.
Cayman Chemical HDAC Fluorometric Activity As
Cayman’s HDAC Fluorometric Activity Assay Kit provides a fast, fluorometric method for measuring HDAC activity without the use of radioactivity, extraction, or chromatography. In the first step, an acetylated lysine substrate is incubated with samples. Deacetylation sensitizes the substrate such that treatment with the HDAC developer in the second step releases a fluorescent product. Trichostatin A is included as a control inhibitor. The fluorophore can be EAsily analyzed using a fluorescence plate reader with an excitation wavelength of 340-360 nm and an emission wavelength of 440-465 nm. The assay can be used for quantifying Class I and II HDAC activity from various sources.
Cayman Chemical cGAS InhIbItr Screeng as 96Wel
Cayman’s cGAS Inhibitor Screening Assay Kit provides a robust and EAsy-to-use platform for identifying novel inhibitors of human cGAS, which is a cytosolic dsDNA sensor that detects microbial pathogens. The assay directly measures 2’3’-cGAMP produced by cGAS in the presence of DNA, ATP, and GTP. The cyclic dinucleotide product of that reaction is quantified via ELISA using a 2’3’-cGAMP-specific antiserum. The cGAS inhibitor CU-76 is included as a positive control. The ELISA portion of Cayman’s cGAS Inhibitor Screening Assay Kit is a competitive assay with a range of 4.57-10,000 pM, a midpoint of approximately 300 pM (50% B/B0), and a sensitivity (80% B/B0) of approximately 50 pM.
Cayman Chemical HemoglobIn ColorImetrIc asy
Hemoglobin (Hb or Hgb) is a globular protein found primarily in erythrocytes that carries oxygen from the lungs to tissue where it releases oxygen and then returns carbon dioxide (CO2) from the tissue to the lungs. Hemoglobin also binds CO2, carbon monoxide (CO), and nitric oxide (NO). Cayman’s Hemoglobin Colorimetric Assay provides a quick, reliable method for determining total hemoglobin concentration in a variety of biological samples, including blood, tissue homogenates and cell lysates. Unlike the universally accepted reference method of hemoglobin determination, which uses potassium cyanide as a reagent and commonly under-estimates hemoglobin levels, Cayman’s Hemoglobin Colorimetric Assay utilizes an optimized detection reagent which is non-toxic, reporting accurate measurements of total hemoglobin concentrations with an absorbance between 560-590 nm.
Cayman Chemical THUNDRcAMP TRFRET asy K 960Wel
A homogeneous competitive TR-FRET immunoassay for quantitative measurement of cAMP in cell lysates
Cayman Chemical sPLA2 asy KIt 96Wel
Cayman’s sPLA2 Assay provides an accurate and convenient method for measurement of sPLA2 activity. This assay uses the 1,2-dithio analog of diheptanoyl phosphatidylcholine which serves as a substrate for most PLA2s with the exception of cPLA2. Upon hydrolysis of the thioester bond at the sn-2 position by PLA2, free thiols are detected using DTNB.